11 October 2011 Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor
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Proceedings Volume 8306, Photonics, Devices, and Systems V; 83060L (2011) https://doi.org/10.1117/12.912264
Event: Photonics Prague 2011, 2011, Prague, Czech Republic
Abstract
Advanced optical instruments are useful for analysis and manipulation of individual living cells and their internal structures. We have employed Raman microspectroscopic analysis for assessment of algal lipid body (LB) volume in vivo. Some algae contain β-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have detected proportionality between the Raman vibrations of β-carotene and the LB volume. This finding may allow fast acquisition of LB volume approximation valuable e.g. for Raman microspectroscopy assisted cell sorting. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based on spectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic manipulations.
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Zdenek Pilát, Zdenek Pilát, Silvie Bernatová, Silvie Bernatová, Jan Ježek, Jan Ježek, Mojmir Šery, Mojmir Šery, Ota Samek, Ota Samek, Pavel Zemánek, Pavel Zemánek, Ladislav Nedbal, Ladislav Nedbal, Martin Trtílek, Martin Trtílek, "Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor", Proc. SPIE 8306, Photonics, Devices, and Systems V, 83060L (11 October 2011); doi: 10.1117/12.912264; https://doi.org/10.1117/12.912264
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