Evaluating the dose effects of a longitudinal micro-CT study on pulmonary tissue in C57BL/6 mice

Sarah A. Detombe; Joy Dunmore-Buyze; Ivailo E. Petrov; Maria Drangova

doi:10.1117/12.912479

9 March 2012 Evaluating the dose effects of a longitudinal micro-CT study on pulmonary tissue in C57BL/6 mice

Sarah A. Detombe, Joy Dunmore-Buyze, Ivailo E. Petrov, Maria Drangova

Proceedings Volume 8313, Medical Imaging 2012: Physics of Medical Imaging; 83130U (2012) https://doi.org/10.1117/12.912479
Event: SPIE Medical Imaging, 2012, San Diego, California, United States

Abstract

Background: Micro-computed tomography offers numerous advantages for small animal imaging, including the ability to monitor the same animals throughout a longitudinal study. However, concerns are often raised regarding the effects of x-ray dose accumulated over the course of the experiment. In this study, we scan C57BL/6 mice multiple times per week for six weeks, to determine the effect of the cumulative dose on pulmonary tissue at the end of the study. Methods/Results: C57BL/6 male mice were split into two groups (irradiated group=10, control group=10). The irradiated group was scanned (80kVp/50mA) each week for 6 weeks; the weekly scan session had three scans. This resulted in a weekly dose of 0.84 Gy, and a total study dose of 5.04 Gy. The control group was scanned on the final week. Scans from weeks 1 and 6 were reconstructed and analyzed: overall, there was no significant difference in lung volume or lung density between the control group and the irradiated group. Similarly, there were no significant differences between the week 1 and week 6 scans in the irradiated group. Histological samples taken from excised lung tissue also showed no evidence of inflammation or fibrosis in the irradiated group. Conclusion: This study demonstrates that a 5 Gy x-ray dose accumulated over six weeks during a longitudinal micro-CT study has no significant effects on the pulmonary tissue of C57BL/6 mice. As a result, the many advantages of micro- CT imaging, including rapid acquisition of high-resolution, isotropic images in free-breathing mice, can be taken advantage of in longitudinal studies without concern for negative dose-related effects.

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Sarah A. Detombe, Joy Dunmore-Buyze, Ivailo E. Petrov, and Maria Drangova "Evaluating the dose effects of a longitudinal micro-CT study on pulmonary tissue in C57BL/6 mice", Proc. SPIE 8313, Medical Imaging 2012: Physics of Medical Imaging, 83130U (9 March 2012); https://doi.org/10.1117/12.912479

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KEYWORDS

Lung

Tissues

X-rays

Image segmentation

Inflammation

Preclinical imaging

Statistical analysis

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