Investigation of the thermally structural denaturation of bovine serum albumin by a home-made optical heterodyne polarimeter

Chien-ming Wu

doi:10.1117/12.905227

15 November 2011 Investigation of the thermally structural denaturation of bovine serum albumin by a home-made optical heterodyne polarimeter

Chien-ming Wu

Author Affiliations +

Proceedings Volume 8321, Seventh International Symposium on Precision Engineering Measurements and Instrumentation; 832135 (2011) https://doi.org/10.1117/12.905227
Event: Seventh International Symposium on Precision Engineering Measurements and Instrumentation, 2011, Yunnan, China

Abstract

Bovine serum albumin (BSA) solutions (2.5 %, PH 7.2) under heat treatment can result in structural changes. Some of the α-helices are transformed at 67°C to random coils resulting in an increased rotation angle. With subsequent heating, the transformed random coils may once again transform to non-native β-sheets and restore the optical rotation angle. These two states are all reversible. However, when the heating temperature goes up to 69°C the denatured BSA starts to transform into a rigid network and becomes an irreversible state. The reversible and irreversible temperatures of BSA were determined and discussed.

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Chien-ming Wu "Investigation of the thermally structural denaturation of bovine serum albumin by a home-made optical heterodyne polarimeter", Proc. SPIE 8321, Seventh International Symposium on Precision Engineering Measurements and Instrumentation, 832135 (15 November 2011); https://doi.org/10.1117/12.905227

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