Characterization of human normal and cancerous gastric submucosa based on multiphoton microscopy

Jiazhao Zhong; G. Chen; J. X. Chen

doi:10.1117/12.920341

13 March 2012 Characterization of human normal and cancerous gastric submucosa based on multiphoton microscopy

Jiazhao Zhong, G. Chen, J. X. Chen

Proceedings Volume 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011); 83290S (2012) https://doi.org/10.1117/12.920341
Event: Photonics and Optoelectronics Meetings 2011, 2011, Wuhan, China

Abstract

Gastric cancer is one of the most frequent cancers in the world; almost two-thirds of gastric cancer cases and deaths occur in less developed regions. The initial diagnosis of gastric cancer often is delayed because up to 80 percent of patients are asymptomatic during the early stages of stomach cancer. So the ability to perform real-time in vivo histological diagnosis for early gastric cancer at the cellular level during ongoing endoscopy is a long-standing goal of endoscopists. In this paper, using multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), MPM images of human normal and cancerous gastric submucosa were obtained at excitation wavelength of 800 nm. The features such as the appearance of abnormal cells and the large loss of collagen in cancerous gastric submucosa were extracted to be as significant indicators to distinguish cancerous submucosa from normal submucosa. With the implementation of multiphoton microscopy concept in endoscopy applications, multiphoton endoscopy might realize in vivo histological diagnosis goal of endoscopists.

Citation Download Citation

Jiazhao Zhong, G. Chen, and J. X. Chen "Characterization of human normal and cancerous gastric submucosa based on multiphoton microscopy", Proc. SPIE 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011), 83290S (13 March 2012); https://doi.org/10.1117/12.920341

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KEYWORDS

Cancer

Endoscopy

In vivo imaging

Multiphoton microscopy

Tissues

Collagen

Second-harmonic generation

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