Abstract
In immunoassay based biosensors development studies polymers, as a matrix, and thiol, amine and aldehyde derivative
compounds, as a antibody linker, are to be experimented. Aim of this study is to test amine and acetate functional group
containing derivatives in liquid phase in order to develop an antibody immobilization strategy for Quartz Crystal
Microbalance (QCM) system. In our study, 4-aminothiophenol (4-AT), carboxylated-PVC (PVC-COOH) and aminated-
PVC (PVC-NH2) compared with each other as a coating material. Surface of the coated AT-cut gold crystals were
characterized with Fourier Transform Infrared spectrometry (FTIR) and Scanning Electron Microscobe (SEM) and
tested in a Bacillus anthracis (GenBank: GQ375871) spores immunoassay model system. Subsequently, a series of SEM
micrographs were taken again in order to investigate surface morphology and show the presence of the B. anthracis
spores on the sensor surface. When experimental results and SEM images were evaluated together, it was suggested that
with the synthesis of PVC like open-chained polymers, containing -NH2 and -SH functional groups, B. anthracis spore
detection can be accomplished on QCM without requiring complicated immobilization procedures and expensive
preliminary preparations.
