16 May 2012 Rapid identification of Yersinia pestis and Brucella melitensis by chip-based continuous flow PCR
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Abstract
To combat the threat of biological agents like Yersinia pestis and Brucella melitensis in bioterroristic scenarios requires fast, easy-to-use and safe identification systems. In this study we describe a system for rapid amplification of specific genetic markers for the identification of Yersinia pestis and Brucella melitensis. Using chip based PCR and continuous flow technology we were able to amplify the targets simultaneously with a 2-step reaction profile within 20 minutes. The subsequent analysis of amplified fragments by standard gel electrophoresis requires another 45 minutes. We were able to detect both pathogens within 75 minutes being much faster than most other nucleic acid amplification technologies.
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Michael Dietzsch, Michael Dietzsch, Nadine Hlawatsch, Nadine Hlawatsch, Falk Melzer, Falk Melzer, Herbert Tomaso, Herbert Tomaso, Claudia Gärtner, Claudia Gärtner, Heinrich Neubauer, Heinrich Neubauer, } "Rapid identification of Yersinia pestis and Brucella melitensis by chip-based continuous flow PCR", Proc. SPIE 8367, Smart Biomedical and Physiological Sensor Technology IX, 83670F (16 May 2012); doi: 10.1117/12.920473; https://doi.org/10.1117/12.920473
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