3D imaging of tomato seeds using frequency domain optical coherence tomography

Chuanmao Fan; Gang Yao

doi:10.1117/12.919347

4 May 2012 3D imaging of tomato seeds using frequency domain optical coherence tomography

Chuanmao Fan, Gang Yao

Proceedings Volume 8369, Sensing for Agriculture and Food Quality and Safety IV; 83690F (2012) https://doi.org/10.1117/12.919347
Event: SPIE Defense, Security, and Sensing, 2012, Baltimore, Maryland, United States

Abstract

A fast imaging system that can reveal internal sample structures is important for research and quality controls of seeds. Optical coherence tomography (OCT) is a non-invasive optical imaging technique that can acquire high speed, high resolution depth-resolved images in scattering samples. It has found numerous applications in studying various biological tissues and other materials in vivo. A few studies have reported the use of OCT in studying seed morphology. However, 3D imaging of internal seed structure has not been reported before. In this study, we used a frequency domain OCT system to image tomato seeds. The system has a central wavelength of 844nm with a 46.8 nm FWHM bandwidth. The requirement for depth scan was eliminated by using a Fourier domain implementation. The B-scan imaging speed was limited by the spectroscopic imaging CCD at 52 kHz. The calibrated system has a 6.7μm depth resolution and a 15.4μm lateral resolution. Our results show that major seed structures can be clearly visualized in OCT images.

Citation Download Citation

Chuanmao Fan and Gang Yao "3D imaging of tomato seeds using frequency domain optical coherence tomography", Proc. SPIE 8369, Sensing for Agriculture and Food Quality and Safety IV, 83690F (4 May 2012); https://doi.org/10.1117/12.919347

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