4 May 2012 Rapid, enhanced detection of Salmonella Typhimurium on fresh spinach leaves using micron-scale, phage-coated magnetoelastic biosensors
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Abstract
In order to cost-effectively and rapidly detect bacterial food contamination in the field, the potential usefulness of phage-coated magnetoelastic (ME) biosensors has been recently reported. These biosensors are freestanding, mass-sensitive biosensors that can be easily batch-fabricated, thereby reducing the fabrication cost per sensor to a fraction of a cent. In addition, the biosensors can be directly placed on fresh produce surfaces and used to rapidly monitor possible bacterial food contamination without any preceding sample preparation. Previous investigations showed that the limit of detection (LOD) with millimeter-scale ME biosensors was fairly low for fresh produce with smooth surfaces (e.g., tomatoes and shell eggs). However, the LOD is anticipated to be dependent on the size of the biosensors as well as the topography of produce surfaces of interest. This paper presents an investigation into the use of micron-scale, phage-coated ME biosensors for the enhanced detection of Salmonella Typhimurium on fresh spinach leaves.
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Shin Horikawa, Shin Horikawa, Kiril A. Vaglenov, Kiril A. Vaglenov, Dana M. Gerken, Dana M. Gerken, Yating Chai, Yating Chai, Mi-Kyung Park, Mi-Kyung Park, Suiqiong Li, Suiqiong Li, Valery A. Petrenko, Valery A. Petrenko, Bryan A. Chin, Bryan A. Chin, } "Rapid, enhanced detection of Salmonella Typhimurium on fresh spinach leaves using micron-scale, phage-coated magnetoelastic biosensors", Proc. SPIE 8369, Sensing for Agriculture and Food Quality and Safety IV, 83690N (4 May 2012); doi: 10.1117/12.920456; https://doi.org/10.1117/12.920456
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