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8 May 2012 Patterned illumination for analysing neuronal function in 3D
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We use patterned 3D multi-spot illumination to perform neuronal multi-site stimulation in rat brain tissue. Using a spatial light modulator, we holograpically project 3D light fields for multi-site two-photon photolysis of caged neurotransmitters to generate synaptic inputs to a neuron. Controlled photostimulation of multiple synapses from various locations in the dendritic tree provides a way to analyze how neurons integrate multiple inputs. Our holographic projection setup is incorporated into a two-photon 3D imaging microscope for visualization and for accurate positioning of specific uncaging sites along the neuron's dendritic tree. We show two-photon images and the neuron's response to holographic photostimulation of synapses along dendrites.
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Mary Ann Go, Christian Stricker, Steve Redman, Hans Bachor, and Vincent Ricardo Daria "Patterned illumination for analysing neuronal function in 3D", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842703 (8 May 2012);

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