The hand-foot-syndrome presents a severe dermal side-effect of chemotherapeutic cancer treatment.
The cause of this side-effect is the elimination of systemically administered chemotherapeutics with
the sweat. Transported to the skin surface, the drugs subsequently penetrate into the skin in the
manner of topically applied substances. Upon accumulation of the chemotherapeutics in the skin the
drugs destroy cells and tissue - in the same way as they are supposed to act in cancer cells.
Aiming at the development of strategies to illuminate the molecular mechanism underlying the handfoot-
syndrome (and, in a second step, strategies to prevent this severe side-effect), it might be
important to evaluate the concentration and distribution of chemotherapeutics and antioxidants in the
human skin. The latter can be estimated by the carotenoid concentration, as carotenoids serve as
marker substances for the dermal antioxidative status.Following the objectives outlined above, this contribution presents a spectroscopic study aiming at
the detection and quantification of carotenoids and selected chemotherapeutics in human skin. To
this end, spontaneous Raman scattering and coherent anti-Stokes Raman scattering (CARS)
microspectroscopy are combined with two-photon excited fluorescence. While the latter technique is
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spectroscopic techniques can - in principle - be applied to any type of analyte molecules.
Furthermore, we will present the monitoring of doxorubicin uptake during experiments.