8 May 2012 Laser heating of gold nanoparticles: photothermal cancer cell therapy
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Abstract
In this work an application of gold nanoparticles in in-vitro photothermal cancer cell therapy is demonstrated. Gold nanoparticles with different diameters - 40, 100 and 200 nm are mixed with HeLa cancer cells. After incubation, the nanoparticles are found to be deposited on the cell's membrane or enter into the cells. Pulsed laser radiation at wavelength of 532 nm delivered by Nd:YAG system is used to irradiate the samples. The experiments are performed at fluences in the range from 50 mJ/cm2 up to the established safety standard for medical lasers of 100 mJ/cm2. The cell viability as a function of the particle dimensions and laser fluence is estimated. The nanoparticles heating and cooling dynamics is traced by a numerical model based on heat diffusion equation combined with Mie theory for calculation of the optical properties of nanoparticles. The particle response to the nanosecond laser heating is investigated experimentally as gold colloids are irradiated at different fluences. The threshold fluences for particle's melting and boiling are defined. We show that at the presented fluence range the particles are decomposed into smaller fragments and even short irradiation time leads to decrease of cell viability.
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N. N. Nedyalkov, P. A. Atanasov, R. A. Toshkova, E. G. Gardeva, L. S. Yossifova, M. T. Alexandrov, D. Karashanova, "Laser heating of gold nanoparticles: photothermal cancer cell therapy", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 84272P (8 May 2012); doi: 10.1117/12.921776; https://doi.org/10.1117/12.921776
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