8 May 2012 Core-shell nanoparticles as enhanced probes for imaging applications
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The development of highly specific markers for fluorescent microscopy has become a very popular research topic. Organic fluorophores have several drawbacks, such as photobleaching and autofluorescence. Therefore increasing interest in inorganic nanoparticles has been observed because of their unseen photostability, chemical robustness and straightforward synthesis. The surface of iron oxide nanoparticles was coated with trialkoxy silanes, which introduced functional groups for possible subsequent coupling reactions. An additional gold layer was added to the surface of the particle to show the enhanced contrast improvement. The nanoparticles were imaged by an optical microscope, in dark field mode, on a glass substrate and inside microorganisms. This proved that the reported method could have great potential as a labelling technique, since it combines the non-photobleaching, photostable nanoparticles with a straightforward and rapid imaging technique.
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Maarten Bloemen, Maarten Bloemen, Ward Brullot, Ward Brullot, Carla Denis, Carla Denis, Louise Vanysacker, Louise Vanysacker, Thierry Verbiest, Thierry Verbiest, } "Core-shell nanoparticles as enhanced probes for imaging applications", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 84272Q (8 May 2012); doi: 10.1117/12.921846; https://doi.org/10.1117/12.921846

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