8 May 2012 In-vivo real-time monitoring of nanoparticle clearance rate from blood circulation using high speed flow cytometry
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Abstract
The rapidly growing application of nanotechnology in medicine has placed new demands on monitoring the clearance rate of various nanoparticles with different shapes, compositions, and conjugations within blood circulation. No clinically relevant method has been developed for rapid and sensitive detection of nanoparticles in blood flow. Our laboratory has developed a laser-based platform with the purpose of in vivo real-time monitoring of nanoparticles using a highly advanced high-speed, multicolor photoacoustic flow cytometry (PAFC). As most nanoparticles have intrinsic absorption, PAFC is an ideal tool for real-time, label-free monitoring of nanoparticle pharmacokinetics. We used four laser wavelengths to verify the concept of in vivo multicolor PAFC, and hypothesize that the potential exists to increase the number of spectral channels. The capability of this platform was demonstrated for detection of magnetic nanobeads and gold nanorods of different sizes and conjugations in blood circulation of animal models. The advantages as well as potential limitations of the new technique are discussed in detail.
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Mustafa Sarimollaoglu, Dmitry A. Nedosekin, Ekaterina I. Galanzha, Vladimir P. Zharov, "In-vivo real-time monitoring of nanoparticle clearance rate from blood circulation using high speed flow cytometry", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 84273R (8 May 2012); doi: 10.1117/12.922808; https://doi.org/10.1117/12.922808
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