Theoretical model of volumetric objects imaging in a microscope

Anton A. Grebenyuk; Vladimir P. Ryabukho

doi:10.1117/12.922198

Abstract

Optical microscope is a well known instrument with many applications in biomedical imaging and precise measurements. Nowadays a lot of modalities arose, which utilize combination of a microscope arrangement with other optical techniques, such as holography or low-coherence interferometry. The most important features of these modalities are related to the diffraction or coherence effects due to such combination, rather than to exact ways of lens aberrations correction. Therefore for analysis and understanding of these effects a theoretical model is necessary, which would be rigorous enough to take into account the diffraction and coherence effects and in the same time simple enough to allow clear physical interpretation of the observed effects. In this paper we propose such a model of volumetric samples imaging in a microscope, that utilizes theory of two-dimensional image formation and analysis of volumetric sample via an "effective" two-dimensional field. Applicability of the proposed model to analysis of volumetric samples imaging is shown by the example of a full-field microscope by Köhler illumination.

Citation Download Citation

Anton A. Grebenyuk, Anton A. Grebenyuk, Vladimir P. Ryabukho, Vladimir P. Ryabukho, } "Theoretical model of volumetric objects imaging in a microscope", Proc. SPIE 8430, Optical Micro- and Nanometrology IV, 84301B (4 May 2012); doi: 10.1117/12.922198; https://doi.org/10.1117/12.922198

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