Using DNA looping to measure sequence dependent DNA elasticity

Alan Kandinov; Krishnan Raghunathan; Jens-Christian Meiners

doi:10.1117/12.945930

10 October 2012 Using DNA looping to measure sequence dependent DNA elasticity

Alan Kandinov, Krishnan Raghunathan, Jens-Christian Meiners

Proceedings Volume 8458, Optical Trapping and Optical Micromanipulation IX; 845816 (2012) https://doi.org/10.1117/12.945930
Event: SPIE NanoScience + Engineering, 2012, San Diego, California, United States

Abstract

We are using tethered particle motion (TPM) microscopy to observe protein-mediated DNA looping in the lactose repressor system in DNA constructs with varying AT / CG content. We use these data to determine the persistence length of the DNA as a function of its sequence content and compare the data to direct micromechanical measurements with constant-force axial optical tweezers. The data from the TPM experiments show a much smaller sequence effect on the persistence length than the optical tweezers experiments.

Citation Download Citation

Alan Kandinov, Krishnan Raghunathan, and Jens-Christian Meiners "Using DNA looping to measure sequence dependent DNA elasticity", Proc. SPIE 8458, Optical Trapping and Optical Micromanipulation IX, 845816 (10 October 2012); https://doi.org/10.1117/12.945930

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