10 October 2012 Extension of the spectral range of bacteriorhodopsin functional activity by energy transfer from quantum dots
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Monodispersed semiconductor nanocrystals or quantum dots (QDs) specifically immobilized on the surface of purple membranes (PMs) containing bacteriorhodopsin (bR) can harvest light in the UV to blue region, which cannot be absorbed efficiently by the PMs alone, and transfer the harvested energy to the retinal chromophores of bR via highly efficient Förster resonance energy transfer (FRET). CdTe or CdSe/ZnS QDs with a quantum yield as high as 70% have been used to estimate different parameters characterizing the improvement of the bR biological function caused by nanocrystals. AFM examination has shown that the most FRET-efficient QD–PM hybrid structures are characterized by the highest level of QD ordering; hence, AFM imaging of bR–PM hybrid materials provides the basis for optimization of the assembly design in order to engineer bio-hybrid structures with advanced optical and photovoltaic properties. Oriented bR-containing proteoliposomes tagged with QDs at a QD-to-bR molar ratio of up to 1:5 have been engineered and used to analyze the photoresponse, with the bR proton pumping considerably increased. Finally, the kinetics of the potential/current generation in films of oriented bR containing or not containing QDs have been analyzed. Incorporation of QDs resulted in an increase in the potential/current generation rate and in an almost fourfold increase in the rate of Mform formation. Thus, the improvement of the bR native function by QDs may be caused by two reasons: an extension of the range of utilized light and an increase in the rate of the bR photocycle.
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Vladimir Oleinikov, Vladimir Oleinikov, Nicolas Bouchonville, Nicolas Bouchonville, Alyona Sukhanova, Alyona Sukhanova, Michael Molinari, Michael Molinari, Svetlana Sizova, Svetlana Sizova, Konstantin Mochalov, Konstantin Mochalov, Anton Chistyakov, Anton Chistyakov, Evgeniy Lukashev, Evgeniy Lukashev, Aliaksandra Rakovich, Aliaksandra Rakovich, John F. Donegan, John F. Donegan, Igor Nabiev, Igor Nabiev, } "Extension of the spectral range of bacteriorhodopsin functional activity by energy transfer from quantum dots", Proc. SPIE 8464, Nanobiosystems: Processing, Characterization, and Applications V, 84640Z (10 October 2012); doi: 10.1117/12.929764; https://doi.org/10.1117/12.929764

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