24 October 2012 A nanofluidic bioarray chip for fast and high-throughput detection of antibodies in biological fluids
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Proceedings Volume 8548, Nanosystems in Engineering and Medicine; 85481Q (2012) https://doi.org/10.1117/12.945970
Event: SPIE Nanosystems in Engineering + Medicine, 2012, Incheon, Korea, Republic of
Abstract
Immunoassays have become a standard in secretome analysis in clinical and research analysis. In this field there is a need for a high throughput method that uses low sample volumes. Microfluidics and nanofluidics have been developed for this purpose. Our lab has developed a nanofluidic bioarray (NBA) chip with the goal being a high throughput system that assays low sample volumes against multiple probes. A combination of horizontal and vertical channels are produced to create an array antigens on the surface of the NBA chip in one dimension that is probed by flowing in the other dimension antibodies from biological fluids. We have tested the NBA chip by immobilizing streptavidin and then biotinylated peptide to detect the presence of a mouse monoclonal antibody (MAb) that is specific for the peptide. Bound antibody is detected by an AlexaFluor 647 labeled goat (anti-mouse IgG) polyclonal antibody. Using the NBA chip, we have successfully detected peptide binding by small-volume (0.5 μl) samples containing 50 attomoles (100 pM) MAb.
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Jonathan Lee, Naveed Gulzar, Jamie K. Scott, Paul C. H. Li, "A nanofluidic bioarray chip for fast and high-throughput detection of antibodies in biological fluids", Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85481Q (24 October 2012); doi: 10.1117/12.945970; https://doi.org/10.1117/12.945970
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