24 October 2012 Optogenetic tools for in vivo applications in neonatal mice
Author Affiliations +
Proceedings Volume 8548, Nanosystems in Engineering and Medicine; 854842 (2012) https://doi.org/10.1117/12.2001205
Event: SPIE Nanosystems in Engineering + Medicine, 2012, Incheon, Korea, Republic of
Spontaneous neural activities exist early in development and their spatiotemporal patterns play important roles in the development of sensory maps such as maps of retinotopy in the visual system. We summarized different optogenetic tools, including transgenic mouse lines, viral-mediated transfection and electroporation methods to enable the expression of light-gated channelrhodopsin (ChR2) in retinal ganglion cells (RGCs) before the onset of vision. Patch-clamp and extracellular recording experiments verified that activities of ChR2-expressing cells were precisely manipulated by the patterns of optical stimuli. In chronic stimulation experiments, light-emitting diodes controlled the activity patterns of ChR2-expressing RGCs in vivo. Changes in the retinotopic map in Superior Colliculus (SC) were examined by quantifying the relative sizes of fluorescently labeled target zones. Our results revealed that various optogenetic and optical tools can manipulate retinal activities with precise temporal patterns. These techniques can be readily used in studying the development of the central nervous system of neonatal rodents.
© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yue Zhang, Yue Zhang, Nan Qin, Nan Qin, Yupu Diao, Yupu Diao, Yangtai Guan, Yangtai Guan, Lu Fan, Lu Fan, Michael C. Crair, Michael C. Crair, Jiayi Zhang, Jiayi Zhang, "Optogenetic tools for in vivo applications in neonatal mice", Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 854842 (24 October 2012); doi: 10.1117/12.2001205; https://doi.org/10.1117/12.2001205


Optogenetic mapping of brain circuitry
Proceedings of SPIE (October 23 2012)
Mathematical model of retina
Proceedings of SPIE (December 26 1996)
Neural Network Approach To Sensory Fusion
Proceedings of SPIE (August 08 1988)

Back to Top