11 December 2012 Motion compensation of optical mapping signals from rat heart slices
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Abstract
Optical mapping is a well established technique for recording monophasic action potential traces either within myocardial slices or on the epicardial surface of isolated hearts. This measuring technique offers a high spatial and sufficient temporal resolution but it is sensitive towards myocardial motion. Motion artifacts occur because the mapping between a certain tissue portion sending out fluorescent light and a pixel of the photodetector changes over time. So far this problem has been adressed in two different ways: Suppressing the motion or ratiometric imaging. Working with beating rat heart slices we developed a different approach to noninvasively record simultaneously optical mapping data and motion of the slices. Our approach is based on image registration. We use the background fluorescent light to perform first a rigid transformation to detect translational and and rotational motion. The contractive motion is recovered using a non-rigid demons registration algorithm afterwards. The investigation is based on recordings of four different slices stained with Di-4-ANEPPS.
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B. Stender, M. Brandenburger, B. Wang, Z. Zhang, A. Schlaefer, "Motion compensation of optical mapping signals from rat heart slices", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855305 (11 December 2012); doi: 10.1117/12.2008992; https://doi.org/10.1117/12.2008992
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