11 December 2012 Motion compensation of optical mapping signals from rat heart slices
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Optical mapping is a well established technique for recording monophasic action potential traces either within myocardial slices or on the epicardial surface of isolated hearts. This measuring technique offers a high spatial and sufficient temporal resolution but it is sensitive towards myocardial motion. Motion artifacts occur because the mapping between a certain tissue portion sending out fluorescent light and a pixel of the photodetector changes over time. So far this problem has been adressed in two different ways: Suppressing the motion or ratiometric imaging. Working with beating rat heart slices we developed a different approach to noninvasively record simultaneously optical mapping data and motion of the slices. Our approach is based on image registration. We use the background fluorescent light to perform first a rigid transformation to detect translational and and rotational motion. The contractive motion is recovered using a non-rigid demons registration algorithm afterwards. The investigation is based on recordings of four different slices stained with Di-4-ANEPPS.
© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
B. Stender, B. Stender, M. Brandenburger, M. Brandenburger, B. Wang, B. Wang, Z. Zhang, Z. Zhang, A. Schlaefer, A. Schlaefer, "Motion compensation of optical mapping signals from rat heart slices", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855305 (11 December 2012); doi: 10.1117/12.2008992; https://doi.org/10.1117/12.2008992

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