Measuring intracellular calcium dynamics of HeLa cells exposed to nitric oxide by microplate fluorescence reader

Yimei Huang; Jiangxu Chen; Hongqin Yang; Liqin Zheng; Yuhua Wang; Hui Li; Shusen Xie

doi:10.1117/12.1000059

11 December 2012 Measuring intracellular calcium dynamics of HeLa cells exposed to nitric oxide by microplate fluorescence reader

Yimei Huang, Jiangxu Chen, Hongqin Yang, Liqin Zheng, Yuhua Wang, Hui Li, Shusen Xie

Author Affiliations +

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 855313 (2012) https://doi.org/10.1117/12.1000059
Event: Photonics Asia, 2012, Beijing, China

Abstract

Nitric oxide (NO) has been reported to have the ability to promote or inhibit the proliferation and metastasis of cancer cells. It appears to have an effect on inducing calcium transient, which participates in essential cellular signaling in the physiological and pathological processes. Our work was intended to study the effects of exogenous NO on intracellular calcium dynamics of HeLa cells with Fluo-3, a calcium fluorescent indicator by microplate fluorescence reader. The results showed that after NO donor was injected into the wells, intracellular Ca²⁺ fluorescence intensity increased significantly compared with that of control group. Furthermore, the calcium transient activated by NO was mainly due to the calcium release from intracellular calcium stores. These would be helpful to further recognize the role of NO involved in cancer cell proliferation and metastasis.

Citation Download Citation

Yimei Huang, Jiangxu Chen, Hongqin Yang, Liqin Zheng, Yuhua Wang, Hui Li, and Shusen Xie "Measuring intracellular calcium dynamics of HeLa cells exposed to nitric oxide by microplate fluorescence reader", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855313 (11 December 2012); https://doi.org/10.1117/12.1000059

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