11 December 2012 Imaging the morphological change of tissue structure during the early phase of esophageal tumor progression using multiphoton microscopy
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Abstract
Esophageal cancer is a common malignancy with a very poor prognosis. Successful strategies for primary prevention and early detection are critically needed to control this disease. Multiphoton microscopy (MPM) is becoming a novel optical tool of choice for imaging tissue architecture and cellular morphology by two-photon excited fluorescence. In this study, we used MPM to image microstructure of human normal esophagus, carcinoma in situ (CIS), and early invasive carcinoma in order to establish the morphological features to differentiate these tissues. The diagnostic features such as the appearance of cancerous cells, the significant loss of stroma, the absence of the basement membrane were extracted to distinguish between normal and cancerous esophagus tissue. These results correlated well with the paired histological findings. With the advancement of clinically miniaturized MPM and the multi-photon probe, combining MPM with standard endoscopy will therefore allow us to make a real-time in vivo diagnosis of early esophageal cancer at the cellular level.
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Jian Xu, Jian Xu, Deyong Kang, Deyong Kang, Meifang Xu, Meifang Xu, Xiaoqin Zhu, Xiaoqin Zhu, Shuangmu Zhuo, Shuangmu Zhuo, Jianxin Chen, Jianxin Chen, } "Imaging the morphological change of tissue structure during the early phase of esophageal tumor progression using multiphoton microscopy", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855334 (11 December 2012); doi: 10.1117/12.999848; https://doi.org/10.1117/12.999848
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