Thermal denaturation of protein studied by terahertz time-domain spectroscopy

Xiuhua Fu; Xiangjun Li; Jianjun Liu; Yong Du; Zhi Hong

doi:10.1117/12.981864

5 December 2012 Thermal denaturation of protein studied by terahertz time-domain spectroscopy

Xiuhua Fu, Xiangjun Li, Jianjun Liu, Yong Du, Zhi Hong

Proceedings Volume 8562, Infrared, Millimeter-Wave, and Terahertz Technologies II; 856218 (2012) https://doi.org/10.1117/12.981864
Event: Photonics Asia, 2012, Beijing, China

Abstract

In this study, the absorption spectra of native or thermal protein were measured in 0.2-1.4THz using terahertz time-domain spectroscopy (THz-TDS) system at room temperature, their absorption spectra and the refractive spectra were obtained. Experimental results indicate that protein both has strong absorption but their characteristics were not distinct in the THz region, and the absorption decreased during thermal denatured state. In order to prove protein had been denatured, we used Differential scanning calorimeter (DSC) measured their denatured temperature, from their DSC heating traces, collagen T^d=101℃, Bovine serum albumin T^d=97℃. While we also combined the Fourier transform infrared spectrometer (FTIR) to investigate their secondary and tertiary structure before and after denatuation, but the results did not have the distinct changes. We turned the absorption spectra and the refractive spectra to the dielectric spectra, and used the one-stage Debye model simulated the terahertz dielectric spectra of protein before and after denaturation. This research proved that the terahertz spectrum technology is feasible in testing protein that were affected by temperature or other factors，which can provide theoretical foundation in the further study about the THz spectrum of protein and peptide temperature stability.

Citation Download Citation

Xiuhua Fu, Xiangjun Li, Jianjun Liu, Yong Du, and Zhi Hong "Thermal denaturation of protein studied by terahertz time-domain spectroscopy", Proc. SPIE 8562, Infrared, Millimeter-Wave, and Terahertz Technologies II, 856218 (5 December 2012); https://doi.org/10.1117/12.981864

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