8 March 2013 Quantitative longitudinal measurement in a rat model of controlled burn severity using spatial frequency domain imaging
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Abstract
Background and Objective: Spatial Frequency Domain Imaging (SFDI) is a non-contact wide-field optical imaging technology currently being developed to investigate the feasibility of quantitative non-invasive evaluation of burn wound severity in a rat model. Our objective is to determine the potential of SFDI for mapping quantitative changes in spatially resolved tissue oxygen saturation and water concentration may be indicative of burn wound severity, healing, and further complications. In this portion of the investigation, we focus on the development of a rat burn model and the acute response of tissue to burn wounds. Study Design/Materials and Methods: A controlled burn protocol involving a heated brass comb was applied to 6 rats. Imaging was acquired at 17 evenly spaced wavelengths in the near-infrared from 650 to 970 nm. Over the course of the 3 hour post-burn period, we were able to map quantitative changes in spatially resolved chromophores. Burn severities were verified post-experiment using standard H and E histology and optical microscopy. Results/Conclusion: In total, we were able to induce 12 superficial-partial thickness burns, 8 deep-partial thickness burns, and 4 full thickness burns in our rat models. While several tissue chromophores were tracked, we found that changes in oxygen saturation and water concentration to be sensitive indicators of burn severity. Future work will include additional longitudinal studies over a period of days in order to investigate which parameters are correlated to tissue healing.
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John Quan Nguyen, Christian Crouzet, Tuan Mai, Kathleen Riola, Daniel Uchitel, Lih-Huei Liaw, Nicole Bernal, Adrien Ponticorvo, Bernard Choi, Anthony J. Durkin, "Quantitative longitudinal measurement in a rat model of controlled burn severity using spatial frequency domain imaging", Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 85650S (8 March 2013); doi: 10.1117/12.2004894; https://doi.org/10.1117/12.2004894
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