Influence of USP laser radiation on cell morphology: HaCat and MG-63 cell lines for bone and soft tissue modelling in dentistry

Joerg Meister; Florian Schelle; Imke Beier; Christoph Bourauel; Matthias Frentzen; Dominik Kraus

doi:10.1117/12.2007559

25 March 2013 Influence of USP laser radiation on cell morphology: HaCat and MG-63 cell lines for bone and soft tissue modelling in dentistry

Joerg Meister, Florian Schelle, Imke Beier, Christoph Bourauel, Matthias Frentzen, Dominik Kraus

Author Affiliations +

Proceedings Volume 8566, Lasers in Dentistry XIX; 85660F (2013) https://doi.org/10.1117/12.2007559
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Due to the high intensities of USP laser radiation, the interaction with matter is always attended with a plasma formation. Therefore the surrounding tissue can be influenced by heat generation and additional light emission from the UV up to the near and mid infrared. In dentistry it is of importance that the treatment of bone and soft tissues, i.e. oral mucosa, with a USP laser should not cause any kind of morphological changes on the cell level leading to a delayed wound healing or cell mutation.

HaCaT keratinocyte cells were used for epidermal (soft tissue) and MG-63 osteoblast-like cells for hard tissue (bone) modelling. Cell growing was realized on glas cover slips. Irradiation was carried out with a USP Nd:YVO4 laser having a center wavelength at 1064 nm. Based on the pulse duration of 8 ps and a pulse repetition rate of 500 kHz the laser emits an average power of 9 W. For efficiency testing of cell removal on glas cover slips, 1, 5, 25, 50 and 75 repetitions of the scanning pattern (scan loops) were used. Heat distribution during laser irradiation was measured with an infrared camera system. Subsequently haematoxylin staining and SEM investigations were used to analyse the morphological changes.

Differences of cell removal efficiency were observed with repetitions ≤25. Irradiated areas with repetitions ≥50 were cell-free. Additionally, repetitions ≥25 showed side effects for both cell lines. Cell destruction in both cell lines could be verified using the haematoxylin staining and the SEM pictures.

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Joerg Meister, Florian Schelle, Imke Beier, Christoph Bourauel, Matthias Frentzen, and Dominik Kraus "Influence of USP laser radiation on cell morphology: HaCat and MG-63 cell lines for bone and soft tissue modelling in dentistry", Proc. SPIE 8566, Lasers in Dentistry XIX, 85660F (25 March 2013); https://doi.org/10.1117/12.2007559

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