13 March 2013 Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green
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Abstract
Our group has constructed a new type of viral nanoparticles (VNPs) from genome-depleted plant infecting brome mosaic virus (BMV) that encapsulates the FDA-approved near infrared (NIR) indocyanine green (ICG)[1]. We refer to these VNPs as optical viral ghosts (OVGs) since the constructs lack the genomic content of wild-type BMV. One of our areas of interest is the application of OVGs for real-time intraoperative NIR fluorescence imaging of small peritoneal ovarian tumor nodules. We target human epidermal growth factor receptor-2 (HER-2) expression in ovarian cancer as a biomarker associated with ovarian cancer, since its over-expression is linked to the disease’s progression to death. We functionalize the OVGs with anti-HER-2 monoclonal antibodies using reductive amination methods. We used fluorescence imaging to visualize the SKOV-3 cells (high HER-2 expression) after incubation with free ICG, OVGs, and functionalized OVGs. Our results suggest the possibility of using anti-HER2 conjugated OVGs in conjunction with cytoreductive surgery to detect small tumor nodules (<5cm) which currently are not excised during surgery.
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Yadir Guerrero, Yadir Guerrero, Baharak Bahmani, Baharak Bahmani, Bonsu Jung, Bonsu Jung, Valentine Vullev, Valentine Vullev, Vikas Kundra, Vikas Kundra, Bahman Anvari, Bahman Anvari, } "Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green", Proc. SPIE 8568, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII, 856819 (13 March 2013); doi: 10.1117/12.2006278; https://doi.org/10.1117/12.2006278
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