5 March 2013 Detection, isolation, and capture of circulating breast cancer cells with photoacoustic flow cytometry
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Abstract
According to the CDC, breast cancer is the most common cancer and the second leading cause of cancer related deaths among women. Metastasis, or the presence of secondary tumors caused by the spread of cancer cells via the circulatory or lymphatic systems, significantly worsens the prognosis of any breast cancer patient. In this study, a technique is developed to detect circulating breast cancer cells in human blood using a photoacoustic flow cytometry method. A Q-switched laser with a 5 ns pulse at 532 nm is used to interrogate thousands of cells with one pulse as they flow through the beam path. Cells which are pigmented, either naturally or artificially, emit an ultrasound wave as a result of the photoacoustic (PA) effect. Breast cancer cells are targeted with chromophores through immunochemistry in order to provide pigment. After which, the device is calibrated to demonstrate a single-cell detection limit. Cultured breast cancer cells are added to whole blood to reach a biologically relevant concentration of about 25-45 breast cancer cells per 1 mL of blood. An in vitro photoacoustic flow cytometer is used to detect and isolate these cells followed by capture with the use of a micromanipulator. This method can not only be used to determine the disease state of the patient and the response to therapy, it can also be used for genetic testing and in vitro drug trials since the circulating cell can be captured and studied.
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Kiran Bhattacharyya, Kiran Bhattacharyya, Martin Njoroge, Martin Njoroge, Benjamin S. Goldschmidt, Benjamin S. Goldschmidt, Brian Gaffigan, Brian Gaffigan, Kyle Rood, Kyle Rood, John A. Viator, John A. Viator, } "Detection, isolation, and capture of circulating breast cancer cells with photoacoustic flow cytometry", Proc. SPIE 8570, Frontiers in Biological Detection: From Nanosensors to Systems V, 85700A (5 March 2013); doi: 10.1117/12.2001744; https://doi.org/10.1117/12.2001744
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