20 March 2013 Towards microscopic resolution in holoscopy
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Abstract
Holoscopy is a new imaging approach combining digital holography and full-field Fourier-domain optical coherence tomography. The interference pattern between light scattered by a sample and a defined reference wave is recorded and processed numerically. During reconstruction numerical refocusing is applied, overcoming the limitation of the focal depth and thus a uniform, diffraction limited lateral resolution over the whole measurement depth can be obtained. The advantage of numerical refocusing becomes especially significant for imaging at high numerical apertures (NAs). We use a high-resolution setup based on a Mach-Zehnder interferometer with an high-resolution microscope objective (NA = 0.75). For reliable reconstruction of a sample volume the Rayleigh length of the microscope objective and the axial resolution, given by the spectral range of the light source, need to be matched. For a 0.75 NA objective a tunable light source with a sweeping range of ! 300nm is required. Here we present as a first step a tunable Ti:sapphire laser with a tuning range of 187 nm. By characterizing the spectral properties of the Ti:sapphire laser and determining the axial point spread function we demonstrate the feasibility of this light source for high-resolution holoscopy.
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Gesa Lilith Franke, Gesa Lilith Franke, Dierck Hillmann, Dierck Hillmann, Christian Lührs, Christian Lührs, Peter Koch, Peter Koch, Jörn Wollenzin, Jörn Wollenzin, Gereon Hüttmann, Gereon Hüttmann, } "Towards microscopic resolution in holoscopy", Proc. SPIE 8571, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII, 85711O (20 March 2013); doi: 10.1117/12.2006806; https://doi.org/10.1117/12.2006806
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