20 March 2013 OCT detection of neural activity in American cockroach nervous system
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Abstract
We show results of a project which focuses on detection of activity in neural tissue with Optical Coherence Tomography (OCT) methods. Experiments were performed in neural cords dissected from the American cockroach (Periplaneta americana L.). Functional OCT imaging was performed with ultrahigh resolution spectral / Fourier domain OCT system (axial resolution 2.5 μm). Electrical stimulation (voltage pulses) was applied to the sensory cercal nerve of the neural cord. Optical detection of functional activation of the sample was performed in the connective between the terminal abdominal ganglion and the fifth abdominal ganglion. Functional OCT data were collected over time with the OCT beam illuminating selected single point in the connectives (i.e. OCT M-scans were acquired). Phase changes of the OCT signal were analyzed to visualize occurrence of activation in the neural cord. Electrophysiology recordings (microelectrode method) were also performed as a reference method to demonstrate electrical response of the sample to stimulation.
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Iwona Gorczyńska, Iwona Gorczyńska, Joanna Wyszkowska, Joanna Wyszkowska, Danuta Bukowska, Danuta Bukowska, Daniel Ruminski, Daniel Ruminski, Karol Karnowski, Karol Karnowski, Maria Stankiewicz, Maria Stankiewicz, Maciej Wojtkowski, Maciej Wojtkowski, } "OCT detection of neural activity in American cockroach nervous system ", Proc. SPIE 8571, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII, 85711V (20 March 2013); doi: 10.1117/12.2007208; https://doi.org/10.1117/12.2007208
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