Sentinel lymph nodes detection with an imaging system using Patent Blue V dye as fluorescent tracer

F. Tellier; J. Steibel; R. Chabrier; J. F. Rodier; G. Pourroy; P. Poulet

doi:10.1117/12.2004067

22 March 2013 Sentinel lymph nodes detection with an imaging system using Patent Blue V dye as fluorescent tracer

F. Tellier, J. Steibel, R. Chabrier, J. F. Rodier, G. Pourroy, P. Poulet

Author Affiliations +

Proceedings Volume 8572, Advanced Biomedical and Clinical Diagnostic Systems XI; 85720K (2013) https://doi.org/10.1117/12.2004067
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Sentinel lymph node biopsy is the gold standard to detect metastatic invasion from primary breast cancer. This method can help patients avoid full axillary chain dissection, thereby decreasing the risk of morbidity. We propose an alternative to the traditional isotopic method, to detect and map the sentinel lymph nodes. Indeed, Patent Blue V is the most widely used dye in clinical routine for the visual detection of sentinel lymph nodes. A Recent study has shown the possibility of increasing the fluorescence quantum yield of Patent Blue V, when it is bound to human serum albumin. In this study we present a preclinical fluorescence imaging system to detect sentinel lymph nodes labeled with this fluorescent tracer. The setup is composed of a black and white CCD camera and two laser sources. One excitation source with a laser emitting at 635 nm and a second laser at 785 nm to illuminate the region of interest. The prototype is operated via a laptop. Preliminary experiments permitted to determine the device sensitivity in the μmol.L^-1 range as regards the detection of PBV fluorescence signals. We also present a preclinical evaluation performed on Lewis rats, during which the fluorescence imaging setup detected the accumulation and fixation of the fluorescent dye on different nodes through the skin.

Citation Download Citation

F. Tellier, F. Tellier, J. Steibel, J. Steibel, R. Chabrier, R. Chabrier, J. F. Rodier, J. F. Rodier, G. Pourroy, G. Pourroy, P. Poulet, P. Poulet, } "Sentinel lymph nodes detection with an imaging system using Patent Blue V dye as fluorescent tracer", Proc. SPIE 8572, Advanced Biomedical and Clinical Diagnostic Systems XI, 85720K (22 March 2013); doi: 10.1117/12.2004067; https://doi.org/10.1117/12.2004067

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $14.40

Non-members: $18.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years