Time reversal optical tomography locates fluorescent targets in a turbid medium

Binlin Wu; W. Cai; S. K. Gayen

doi:10.1117/12.2002898

13 March 2013 Time reversal optical tomography locates fluorescent targets in a turbid medium

Binlin Wu, W. Cai, S. K. Gayen

Proceedings Volume 8574, Multimodal Biomedical Imaging VIII; 85740N (2013) https://doi.org/10.1117/12.2002898
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

A fluorescence optical tomography approach that extends time reversal optical tomography (TROT) to locate fluorescent targets embedded in a turbid medium is introduced. It uses a multi-source illumination and multi-detector signal acquisition scheme, along with TR matrix formalism, and multiple signal classification (MUSIC) to construct pseudo-image of the targets. The samples consisted of a single or two small tubes filled with water solution of Indocyanine Green (ICG) dye as targets embedded in a 250 mm × 250 mm × 60 mm rectangular cell filled with Intralipid-20% suspension as the scattering medium. The ICG concentration was 1μM, and the Intralipid-20% concentration was adjusted to provide ~ 1-mm transport length for both excitation wavelength of 790 nm and fluorescence wavelength around 825 nm. The data matrix was constructed using the diffusely transmitted fluorescence signals for all scan positions, and the TR matrix was constructed by multiplying data matrix with its transpose. A pseudo spectrum was calculated using the signal subspace of the TR matrix. Tomographic images were generated using the pseudo spectrum. The peaks in the pseudo images provided locations of the target(s) with sub-millimeter accuracy. Concurrent transmission TROT measurements corroborated fluorescence-TROT findings. The results demonstrate that TROT is a fast approach that can be used to obtain accurate three-dimensional position information of fluorescence targets embedded deep inside a highly scattering medium, such as, a contrast-enhanced tumor in a human breast.

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Binlin Wu, W. Cai, and S. K. Gayen "Time reversal optical tomography locates fluorescent targets in a turbid medium", Proc. SPIE 8574, Multimodal Biomedical Imaging VIII, 85740N (13 March 2013); https://doi.org/10.1117/12.2002898

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KEYWORDS

Luminescence

Target detection

Optical tomography

Scattering

Sensors

Breast

Absorption

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