13 March 2013 Multicolor probe-based confocal laser endomicroscopy: a new world for in vivo and real-time cellular imaging
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Proceedings Volume 8575, Endoscopic Microscopy VIII; 857504 (2013) https://doi.org/10.1117/12.2002490
Event: SPIE BiOS, 2013, San Francisco, California, United States
Since its inception in the field of in vivo imaging, endomicroscopy through optical fiber bundles, or probe-based Confocal Laser Endomicroscopy (pCLE), has extensively proven the benefit of in situ and real-time examination of living tissues at the microscopic scale. By continuously increasing image quality, reducing invasiveness and improving system ergonomics, Mauna Kea Technologies has turned pCLE not only into an irreplaceable research instrument for small animal imaging, but also into an accurate clinical decision making tool with applications as diverse as gastrointestinal endoscopy, pulmonology and urology. The current implementation of pCLE relies on a single fluorescence spectral band making different sources of in vivo information challenging to distinguish. Extending the pCLE approach to multi-color endomicroscopy therefore appears as a natural plan. Coupling simultaneous multi-laser excitation with minimally invasive, microscopic resolution, thin and flexible optics, allows the fusion of complementary and valuable biological information, thus paving the way to a combination of morphological and functional imaging. This paper will detail the architecture of a new system, Cellvizio Dual Band, capable of video rate in vivo and in situ multi-spectral fluorescence imaging with a microscopic resolution. In its standard configuration, the system simultaneously operates at 488 and 660 nm, where it automatically performs the necessary spectral, photometric and geometric calibrations to provide unambiguously co-registered images in real-time. The main hardware and software features, including calibration procedures and sub-micron registration algorithms, will be presented as well as a panorama of its current applications, illustrated with recent results in the field of pre-clinical imaging.
© (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Tom Vercauteren, Tom Vercauteren, François Doussoux, François Doussoux, Matthieu Cazaux, Matthieu Cazaux, Guillaume Schmid, Guillaume Schmid, Nicolas Linard, Nicolas Linard, Marie-Amélie Durin, Marie-Amélie Durin, Hédi Gharbi, Hédi Gharbi, François Lacombe, François Lacombe, } "Multicolor probe-based confocal laser endomicroscopy: a new world for in vivo and real-time cellular imaging", Proc. SPIE 8575, Endoscopic Microscopy VIII, 857504 (13 March 2013); doi: 10.1117/12.2002490; https://doi.org/10.1117/12.2002490

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