Label free multiphoton imaging of human pulmonary tissues through two-meter-long microstructured fiber and multicore image-guide

G. Ducourthial; C. Lefort; D. A. Peyrot; T. Mansuryan; S. G. Kruglik; C. Vever-Bizet; L. Thiberville; F. Lacombe; Geneviève Bourg-Heckly; Frédéric Louradour

doi:10.1117/12.2003118

Translator Disclaimer

13 March 2013 Label free multiphoton imaging of human pulmonary tissues through two-meter-long microstructured fiber and multicore image-guide

G. Ducourthial, C. Lefort, D. A. Peyrot, T. Mansuryan, S. G. Kruglik, C. Vever-Bizet, L. Thiberville, F. Lacombe, Geneviève Bourg-Heckly, Frédéric Louradour

Author Affiliations +

Proceedings Volume 8575, Endoscopic Microscopy VIII; 85750H (2013) https://doi.org/10.1117/12.2003118
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

This work deals with label free multiphoton imaging of the human lung tissue extra-cellular matrix (ECM) through optical fibers. Two devices were developed, the first one using distal scanning associated to a double clad large mode area (LMA) air-silica microstructured fiber, the second one using proximal scanning of a miniature multicore image guide (30000 cores inside a 0.8 mm diameter). In both cases, the main issue has been efficient linear and nonlinear distortion pre-compensation of excitation pulses. By inserting before the delivery fiber a compact (10 cm × 10 cm footprint) grisms-based stretcher (a grating in close contact with a prism) made of readily available commercial components, we achieved as short as 35-femtosecond-duration pulses that were temporally compressed at the direct exit of a 2-meter-long fiber. Interestingly, this femtosecond pulse fiber delivery device is also wavelength tunable over more than 100 nm inside the Ti: Sapphire emission band. With the help of distal scan system, those unique features allowed us to record elastin (through two-photon fluorescence) and collagen (through second harmonic generation) fibered network images. These images were obtained ex-vivo with only 15 mW @ 80 MHz of IR radiation delivered to the alveoli or bronchus tissues. 3D imaging with 400-μm-penetration depth inside the tissue was possible working with a 2-meter-long LMA fiber. With the help of proximal scanning, the miniature image guide allowed us to perform endoscopic real time microimaging of the ECM ex vivo.

Citation Download Citation

G. Ducourthial, C. Lefort, D. A. Peyrot, T. Mansuryan, S. G. Kruglik, C. Vever-Bizet, L. Thiberville, F. Lacombe, Geneviève Bourg-Heckly, and Frédéric Louradour "Label free multiphoton imaging of human pulmonary tissues through two-meter-long microstructured fiber and multicore image-guide", Proc. SPIE 8575, Endoscopic Microscopy VIII, 85750H (13 March 2013); https://doi.org/10.1117/12.2003118

ACCESS THE FULL ARTICLE