Microstructured optical fiber Bragg grating sensor for DNA detection

A. Candiani; S. Giannetti; M. Sozzi; E. Coscelli; F. Poli; A. Cucinotta; A. Bertucci; R. Corradini; M. Konstantaki; W. Margulis; S. Pissadakis; S. Selleri

doi:10.1117/12.2003997

20 March 2013 Microstructured optical fiber Bragg grating sensor for DNA detection

A. Candiani, S. Giannetti, M. Sozzi, E. Coscelli, F. Poli, A. Cucinotta, A. Bertucci, R. Corradini, M. Konstantaki, W. Margulis, S. Pissadakis, S. Selleri

Author Affiliations +

Proceedings Volume 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII; 85760E (2013) https://doi.org/10.1117/12.2003997
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

In this work the inner surface of a microstructured optical fiber, where a Bragg grating was previously inscribed, has been functionalized using peptide nucleic acid probe targeting a DNA sequence of the cystic fibrosis disease. The solution of DNA molecules, matched with the PNA probes, has been infiltrated inside the fiber capillaries and hybridization has been realized according to the Watson - Crick Model. In order to achieve signal amplification, oligonucleotide-functionalized gold nanoparticles were then infiltrated and used to form a sandwich-like system. Experimental measurements show a clear wavelength shift of the reflected high order mode for a 100 nM DNA solution. Several experiments have been carried out on the same fiber using the identical concentration, showing the same modulation and proving a good reproducibility of the results, suggesting the possibility of the reuse of the sensor. Measurements have been also made using a 100 nM mis-matched DNA solution, containing a single nucleotide polymorphism, demonstrating the high selectivity of the sensor.

Citation Download Citation

A. Candiani, S. Giannetti, M. Sozzi, E. Coscelli, F. Poli, A. Cucinotta, A. Bertucci, R. Corradini, M. Konstantaki, W. Margulis, S. Pissadakis, and S. Selleri "Microstructured optical fiber Bragg grating sensor for DNA detection ", Proc. SPIE 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII, 85760E (20 March 2013); https://doi.org/10.1117/12.2003997

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available