20 March 2013 Whispering gallery mode aptasensors for detection of blood proteins
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Abstract
Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was numerically modelled and optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system.
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L. Pasquardini, L. Pasquardini, S. Berneschi, S. Berneschi, A. Barucci, A. Barucci, F. Cosi, F. Cosi, M. Insinna, M. Insinna, L. Lunelli, L. Lunelli, G. Nunzi Conti, G. Nunzi Conti, C. Pederzolli, C. Pederzolli, S. Salvadori, S. Salvadori, S. Soria, S. Soria, } "Whispering gallery mode aptasensors for detection of blood proteins", Proc. SPIE 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII, 85760U (20 March 2013); doi: 10.1117/12.2001987; https://doi.org/10.1117/12.2001987
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