Steady state and time-resolved fluorescence spectroscopic characterisation of normal and cancerous urine

Ramu Rajasekaran; Prakasa Rao Aruna; Munusamy Balu David; Dornadula Koteeswaran; Kulandaivel Muthuvelu; Rai R.; Singaravelu Ganesan

doi:10.1117/12.2006086

19 March 2013 Steady state and time-resolved fluorescence spectroscopic characterization of normal and cancerous urine

Ramu Rajasekaran, Prakasa Rao Aruna, Munusamy Balu David, Dornadula Koteeswaran, Kulandaivel Muthuvelu, Rai R., Singaravelu Ganesan

Author Affiliations +

Proceedings Volume 8577, Optical Biopsy XI; 857707 (2013) https://doi.org/10.1117/12.2006086
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Urine is one of the diagnostically important bio fluids, as it has many metabolites and some of them are native fluorophores. There may be a variation in the distribution and the physiochemical properties of the fluorophores during any metabolic change and pathologic conditions. Native fluorescence spectroscopy has been considered as a promising tool to characterize the fluorophores present in the urine. In this study, we aimed at characterizing the urine of both normal and patients with confirmed cancer using steady state and time-resolved fluorescence spectroscopy at 280 nm and 350 nm excitation. It is observed that the metabolites indoxyl sulphate and neopterin and its derivatives are responsible for altered spectral signatures at 280 nm, and 350 nm excitation. The overall spectral data were subjected to Principal Component Analysis and the resultant components were used as input in the linear discriminant analysis. As a total, 84% and 81.8% of samples were correctly classified at 280 nm and 350 nm respectively.

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Ramu Rajasekaran, Prakasa Rao Aruna, Munusamy Balu David, Dornadula Koteeswaran, Kulandaivel Muthuvelu, Rai R., and Singaravelu Ganesan "Steady state and time-resolved fluorescence spectroscopic characterization of normal and cancerous urine", Proc. SPIE 8577, Optical Biopsy XI, 857707 (19 March 2013); https://doi.org/10.1117/12.2006086

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