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19 March 2013Investigation of native fluorescence spectral difference among prostate cancer cell lines with different risk levels
Yang Pu,1 Jianpeng Xue,2,3 Baogang Xu,3 Wubao Wang,1 Yueqing Gu,2 Rui Tang,3 S. Achilefu,3 Ellen Ackerstaff,4 Jason A. Koutcher,4 R. R. Alfano1
1The City College of New York (United States) 2China Pharmaceutical Univ. (China) 3Washington Univ. School of Medicine in St. Louis (United States) 4Memorial Sloan-Kettering Cancer Ctr. (United States)
The alteration of native fluorophores among different types of cancer cell lines was investigated by the fluorescence spectroscopy. Different types of cancer cell lines with different risk levels, such as moderate metastatic (DU-145) and advanced metastatic (PC-3) cell lines as well as normal cell line (Fibroblast), were excited by the selective excitation wavelength of 300 nm to explore changes of the relative contents of tryptophan and NADH using principal component analysis (PCA). The higher relative content of tryptophan was observed in the advanced metastatic cancer cell lines in comparison with the moderate metastatic and non aggressive cell lines.
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Yang Pu, Jianpeng Xue, Baogang Xu, Wubao Wang, Yueqing Gu, Rui Tang, S. Achilefu, Ellen Ackerstaff, Jason A. Koutcher, R. R. Alfano, "Investigation of native fluorescence spectral difference among prostate cancer cell lines with different risk levels," Proc. SPIE 8577, Optical Biopsy XI, 85770X (19 March 2013);