25 March 2013 Near-infrared spectroscopy of renal tissue in vivo
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Abstract
We have developed a method to quantify hemoglobin concentration and oxygen saturation within the renal cortex by near-infrared spectroscopy. A fiber optic probe was used to transmit the radiation of three semiconductor lasers at 690 nm, 800 nm and 830 nm to the tissue, and to collect diffusely remitted light at source-detector separations from 1 mm to 4 mm. To derive tissue hemoglobin concentration and oxygen saturation of hemoglobin the spatial dependence of the measured cw intensities was fitted by a Monte Carlo model. In this model the tissue was assumed to be homogeneous. The scaling factors between measured intensities and simulated photon flux were obtained by applying the same setup to a homogeneous semi-infinite phantom with known optical properties and by performing Monte Carlo simulations for this phantom. To accelerate the fit of the tissue optical properties a look-up table of the simulated reflected intensities was generated for the needed range of absorption and scattering coefficients. The intensities at the three wavelengths were fitted simultaneously using hemoglobin concentration, oxygen saturation, the reduced scattering coefficient at 800 nm and the scatter power coefficient as fit parameters. The method was employed to study the temporal changes of renal hemoglobin concentration and blood oxygenation on an anesthetized rat during a short period of renal ischemia induced by aortic occlusion and during subsequent reperfusion.
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Dirk Grosenick, Dirk Grosenick, Oliver Steinkellner, Oliver Steinkellner, Heidrun Wabnitz, Heidrun Wabnitz, Rainer Macdonald, Rainer Macdonald, Thoralf Niendorf, Thoralf Niendorf, Kathleen Cantow, Kathleen Cantow, Bert Flemming, Bert Flemming, Erdmann Seeliger, Erdmann Seeliger, } "Near-infrared spectroscopy of renal tissue in vivo", Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 85781P (25 March 2013); doi: 10.1117/12.2000409; https://doi.org/10.1117/12.2000409
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