25 March 2013 A dual-reporter fluorescent imaging approach can be used to estimate sentinel lymph node tumor burden
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Abstract
The presence of metastatic tumor cells in tumor-draining lymph nodes is an important indicator for cancer staging and therapy. Current clinical approaches of assessing lymph node tumor burden require invasive surgery that can be associated with nerve damage and other complications. In this study, a dual-reporter fluorescence molecular imaging approach, previously validated for quantifying targeted reporter binding in various human tumor xenographs, was assessed as a means of quantifying tumor burden in metastatic disease in mice. The utility of the dual-reporter imaging approach to measure tumor burden in sentinel lymph nodes was investigated in a bioluminescent human breast cancer xenograph model in 18 female nude mice. Once the presence of tumor in the lymph node was confirmed by bioluminescent imaging, fluorescently labeled anti-EGFR antibody and an untargeted antibody (labeled with a different fluorophore) were injected intradermally, proximal to the lymph node, and the uptake of the two reporters was imaged simultaneously with a with a flat-panel fluorescent scanner. Preliminary results demonstrated a statistically significant correlation between the dual-reporter measured tumor burden and the bioluminescent measure of tumor burden.
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Kenneth M. Tichauer, Kenneth M. Tichauer, Kimberley S. Samkoe, Kimberley S. Samkoe, Jason R. Gunn, Jason R. Gunn, Ramesh Govindan, Ramesh Govindan, Aravind Viswanathan, Aravind Viswanathan, P. Jack Hoopes, P. Jack Hoopes, Tayyaba Hasan, Tayyaba Hasan, Peter A. Kaufman, Peter A. Kaufman, Brian W. Pogue, Brian W. Pogue, } "A dual-reporter fluorescent imaging approach can be used to estimate sentinel lymph node tumor burden", Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 85781V (25 March 2013); doi: 10.1117/12.2004913; https://doi.org/10.1117/12.2004913
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