Fluorescence imaging of vascular endothelial growth factor in mice tumors using targeted liposome ICG probe

Saeid Zanganeh; Yan Xu; Marina V. Backer; Joseph M. Backer; Quing Zhu

doi:10.1117/12.2007296

25 March 2013 Fluorescence imaging of vascular endothelial growth factor in mice tumors using targeted liposome ICG probe

Saeid Zanganeh, Yan Xu, Marina V. Backer, Joseph M. Backer, Quing Zhu

Author Affiliations +

Proceedings Volume 8578, Optical Tomography and Spectroscopy of Tissue X; 85782O (2013) https://doi.org/10.1117/12.2007296
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Indocyanine Green encapsulating liposomes (Lip/ICG) and scVEGF-Lip/ICG liposomes, decorated with site-specifically lipidated engineered single-chain vascular endothelial growth factor (scVEGF) for targeting VEGF receptors were tested as potential tracers for fluorescent tomography. Two groups of experiments were conducted with tumor-bearing mice (n=4 to 6 per group) with tumors placed in a scattering medium at the imaging depths of 1.5 and 2.0 cm. Lip/ICG and scVEGF-Lip/ICG were injected intravenously in the amounts corresponding to 5 nmol of ICG/mouse. We detected kinetics of increase and decline in fluorescent signals in tumors for both imaging depths and for both targeted and untargeted Lip/ICG. Maximum fluorescent signals were approximately 2-fold higher at 1.5 cm vs. 2.0 cm imaging. A signal from untargeted Lip/ICG reached maximum at 15 min post-injection and then rapidly declined with t_1/2 ~15 min. In contrast, a signal from targeted scVEGF-Lip/ICG reached maximum at 30 min post-injection and then slow declined with t1/2 ~60-90 min. Preferential retention of scVEGF-Lip(ICG) vs. Lip(ICG) was confirmed by the analysis of fluorescence in cryosections of corresponding tumors, harvested at 400 min post-injection. Our results suggest that targeted scVEGF-Lip/ICG can provide for significantly better post-injection time window for detection of relatively deeply seated tumors.

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Saeid Zanganeh, Yan Xu, Marina V. Backer, Joseph M. Backer, and Quing Zhu "Fluorescence imaging of vascular endothelial growth factor in mice tumors using targeted liposome ICG probe ", Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 85782O (25 March 2013); https://doi.org/10.1117/12.2007296

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