25 March 2013 Fluorescence imaging of vascular endothelial growth factor in mice tumors using targeted liposome ICG probe
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Abstract
Indocyanine Green encapsulating liposomes (Lip/ICG) and scVEGF-Lip/ICG liposomes, decorated with site-specifically lipidated engineered single-chain vascular endothelial growth factor (scVEGF) for targeting VEGF receptors were tested as potential tracers for fluorescent tomography. Two groups of experiments were conducted with tumor-bearing mice (n=4 to 6 per group) with tumors placed in a scattering medium at the imaging depths of 1.5 and 2.0 cm. Lip/ICG and scVEGF-Lip/ICG were injected intravenously in the amounts corresponding to 5 nmol of ICG/mouse. We detected kinetics of increase and decline in fluorescent signals in tumors for both imaging depths and for both targeted and untargeted Lip/ICG. Maximum fluorescent signals were approximately 2-fold higher at 1.5 cm vs. 2.0 cm imaging. A signal from untargeted Lip/ICG reached maximum at 15 min post-injection and then rapidly declined with t1/2 ~15 min. In contrast, a signal from targeted scVEGF-Lip/ICG reached maximum at 30 min post-injection and then slow declined with t1/2 ~60-90 min. Preferential retention of scVEGF-Lip(ICG) vs. Lip(ICG) was confirmed by the analysis of fluorescence in cryosections of corresponding tumors, harvested at 400 min post-injection. Our results suggest that targeted scVEGF-Lip/ICG can provide for significantly better post-injection time window for detection of relatively deeply seated tumors.
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Saeid Zanganeh, Saeid Zanganeh, Yan Xu, Yan Xu, Marina V. Backer, Marina V. Backer, Joseph M. Backer, Joseph M. Backer, Quing Zhu, Quing Zhu, } "Fluorescence imaging of vascular endothelial growth factor in mice tumors using targeted liposome ICG probe ", Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 85782O (25 March 2013); doi: 10.1117/12.2007296; https://doi.org/10.1117/12.2007296
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