15 February 2013 Raman fiberoptic probe for monitoring human tissue engineered oral mucosa constructs
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Abstract
In oral and maxillofacial surgery, there is a need for tissue engineered constructs for dental implants, reconstructions due to trauma, oral cancer or congenital defects. A non-invasive quality monitoring of the fabrication of tissue engineered constructs during their production and implantation is a required component of any successful tissue engineering technique. We demonstrate the design and application of a Raman spectroscopic probe for rapid and noninvasive monitoring of Ex Vivo Produced Oral Mucosa Equivalent constructs (EVPOMEs). We conducted in vivo studies to identify Raman spectroscopic failure indicators for EVPOMEs (already developed in vitro), and found that Raman spectra of EVPOMEs exposed to thermal stress showed correlation of the band height ratio of CH2 deformation to phenylalanine ring breathing modes, providing a Raman metric to distinguish between viable and nonviable constructs. This is the first step towards the ultimate goal to design a stand-alone system, which will be usable in a clinical setting, as the data processing and analysis will be performed with minimal user intervention, based on already established and tested Raman spectroscopic indicators for EVPOMEs.
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Alexander Khmaladze, Alexander Khmaladze, Shiuhyang Kuo, Shiuhyang Kuo, Paul Okagbare, Paul Okagbare, Cynthia L. Marcelo, Cynthia L. Marcelo, Stephen E. Feinberg, Stephen E. Feinberg, Michael D. Morris, Michael D. Morris, } "Raman fiberoptic probe for monitoring human tissue engineered oral mucosa constructs", Proc. SPIE 8579, Optical Interactions with Tissue and Cells XXIV, 85790L (15 February 2013); doi: 10.1117/12.2005546; https://doi.org/10.1117/12.2005546
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