1 March 2013 Enhancing antibiofilm efficacy in antimicrobial photodynamic therapy: effect of microbubbles
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Abstract
In this study, we tested the hypothesis that a microbubble containing photosensitizer when activated with light would enable comprehensive disinfection of bacterial biofilms in infected root dentin by antimicrobial photodynamic therapy (APDT). Experiments were conducted in two stages. In the stage-1, microbubble containing photosensitizing formulation was tested for its photochemical properties. In the stage-2, the efficacy of microbubble containing photosensitizing formulation was tested on in vitro infected root canal model, developed with monospecies biofilm models of Enterococcus faecalis on root dentin substrate. The findings from this study showed that the microbubble containing photosensitizing formulation was overall the most effective formulation for photooxidation, generation of singlet oxygen, and in disinfecting the biofilm bacteria in the infected root canal model. This modified photosensitizing formulation will have potential advantages in eliminating bacterial biofilms from infected root dentin.
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Anil Kishen, Saji George, "Enhancing antibiofilm efficacy in antimicrobial photodynamic therapy: effect of microbubbles", Proc. SPIE 8580, Dynamics and Fluctuations in Biomedical Photonics X, 85800B (1 March 2013); doi: 10.1117/12.2005908; https://doi.org/10.1117/12.2005908
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