4 March 2013 Förster resonance energy transfer photoacoustic microscopy
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Förster resonance energy transfer (FRET) provides fluorescence signals sensitive to intra- and inter-molecular distances in the 1-10 nm range. Widely applied in the optical imaging environment, FRET enables visualization of physicochemical processes in molecular interactions and conformation changes. We reported photoacoustic imaging of FRET, based on non-radiative decay that produces heat and subsequent acoustic waves. The experimental results show that photoacoustic imaging offers better penetration into scattering biological tissue. Through its ability to three-dimensionally image tissue with scalable resolution, photoacoustic microscopy provides a beneficial biomedical tool to broaden the in vivo application of the FRET technique.
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Yu Wang, Yu Wang, Lihong V. Wang, Lihong V. Wang, } "Förster resonance energy transfer photoacoustic microscopy", Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 85812E (4 March 2013); doi: 10.1117/12.2004150; https://doi.org/10.1117/12.2004150

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