4 March 2013 Viewing individual cells and ambient microvasculature using two molecular contrasts
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Abstract
To view the individual cells and ambient microvasculature simultaneously will be helpful to study tumor angiogenesis and microenvironments. To achieve this, two molecular contrast mechanisms were exploited simultaneously by integrating two imaging modalities, confocal fluorescence microscopy (CFM) and photoacoustic microscopy (PAM). These share the same scanning optical path and laser source. The induced photoacoustic (PA) signal was detected by a highly sensitive needle hydrophone; while the back-traveling fluorescent photons emitted from the same sample were collected by an avalanche photodetector. Experiments on ex vivo rat bladders were conducted. The CFM image depicted the shape and size of the individual cells successfully. Besides large polygonal umbrella cells, some intracellular components can also be discerned. With the CFM image presenting morphologic cellular information in the bladder wall, the PAM image provides the complementary information, based on the endogenous optical absorption contrast, of the microvascular distribution inside the bladder wall, from large vessels to capillaries. Such multimodal imaging provides the opportunity to realize both histological assay and characterization of microvasculature using one imaging setup. This approach offers the possibility of comprehensive diagnosis of cancer in vivo.
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Zhixing Xie, Zhixing Xie, Sung-Liang Chen, Sung-Liang Chen, Mario L. Fabiilli, Mario L. Fabiilli, J. Brian Fowlkes, J. Brian Fowlkes, K. Kirk Shung, K. Kirk Shung, Qifa Zhou, Qifa Zhou, Xunbin Wei, Xunbin Wei, Paul L. Carson, Paul L. Carson, Xueding Wang, Xueding Wang, } "Viewing individual cells and ambient microvasculature using two molecular contrasts", Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 85813G (4 March 2013); doi: 10.1117/12.2004666; https://doi.org/10.1117/12.2004666
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