Objective evaluation of the microscopic image sharpness for diagnostic metaphase chromosomes

Yuchen Qiu; Yuhua Li; Liqiang Ren; Wei R. Chen; Bin Zheng; Shibo Li; Hong Liu

doi:10.1117/12.2001533

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21 February 2013 Objective evaluation of the microscopic image sharpness for diagnostic metaphase chromosomes

Yuchen Qiu, Yuhua Li, Liqiang Ren, Wei R. Chen, Bin Zheng, Shibo Li, Hong Liu

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Proceedings Volume 8582, Biophotonics and Immune Responses VIII; 85820I (2013) https://doi.org/10.1117/12.2001533
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The purpose of this study is to objectively investigate image sharpness of the metaphase chromosomes at different scanning speeds. In cytogenetic imaging, high scanning speed is sometimes applied to optimize the efficiency. However, at high scanning speed, the obtained image may be deteriorated, due to the scanning blur, under exposure and random vibration of the scanning stage. In this study, the image sharpness of metaphase chromosomes is objectively evaluated. A standard resolution target and several metaphase chromosomes are first imaged under different speeds, by a prototype scanning microscope. Then the sharpness of the acquired images is objectively assessed using a sharpness function. For this prototype system, the results demonstrate that the image sharpness of the metaphase chromosomes is optimized when the scanning speed is 0.8mm/s, under the specified experimental conditions. The results of this study may be useful for optimizing the efficiency and quality of clinical cytogenetic imaging procedures.

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Yuchen Qiu, Yuhua Li, Liqiang Ren, Wei R. Chen, Bin Zheng, Shibo Li, and Hong Liu "Objective evaluation of the microscopic image sharpness for diagnostic metaphase chromosomes", Proc. SPIE 8582, Biophotonics and Immune Responses VIII, 85820I (21 February 2013); https://doi.org/10.1117/12.2001533

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