A bispecific peptide based near-infrared probe for in vivo tumor diagnosis

Li Ding; Wei R. Chen; Yueqing Gu

doi:10.1117/12.2003242

22 February 2013 A bispecific peptide based near-infrared probe for in vivo tumor diagnosis

Li Ding, Wei R. Chen, Yueqing Gu

Proceedings Volume 8582, Biophotonics and Immune Responses VIII; 85820T (2013) https://doi.org/10.1117/12.2003242
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The epidermal growth factor receptor EGFR and HER2 are members of recepeter tyrosine kinase family. Overexpression of EGFR and HER2 has been observed in a variety of human tumors, making these receptors promising targets for tumor diagnosis. An affibody targeting HER2 and a nanobody targeting EGFR were reported before. In this Manuscript, we described an bispecific peptide combined with an affibody and a nanonbody through a linker―（G4S）3 . And the bispecific peptide was labeled with near-infrared (NIR) fluorochrome ICG-Der-02 for in vivo tumor EGFR and HER2 targeting. Afterwards, the EGFR and HER2 specificity of the fluorescent probe was tested in vitro for receptor binding assay and fluorescence microscopy and in vivo for subcutaneous MDA-MB-231 tumor targeting. The results indicated that the bispecific peptide had a high affinity to EGFR and HER2. Besides, in vitro and in vivo tumor targeting experiment indicated that the ICG-Der-02-( bispecific peptide) showed excellent tumor activity accumulation. Noninvasive NIR fluorescence imaging is able to detect tumor EGFR and HER2 expression based upon the highly potent bispecific peptide probe.

Citation Download Citation

Li Ding, Wei R. Chen, and Yueqing Gu "A bispecific peptide based near-infrared probe for in vivo tumor diagnosis", Proc. SPIE 8582, Biophotonics and Immune Responses VIII, 85820T (22 February 2013); https://doi.org/10.1117/12.2003242

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Tumors

In vivo imaging

Near infrared

Luminescence

In vitro testing

Receptors

Proteins

Show All Keywords

Keywords/Phrases

Search In:

Publication Years