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22 February 2013 A bispecific peptide based near-infrared probe for in vivo tumor diagnosis
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Proceedings Volume 8582, Biophotonics and Immune Responses VIII; 85820T (2013)
Event: SPIE BiOS, 2013, San Francisco, California, United States
The epidermal growth factor receptor EGFR and HER2 are members of recepeter tyrosine kinase family. Overexpression of EGFR and HER2 has been observed in a variety of human tumors, making these receptors promising targets for tumor diagnosis. An affibody targeting HER2 and a nanobody targeting EGFR were reported before. In this Manuscript, we described an bispecific peptide combined with an affibody and a nanonbody through a linker―(G4S)3 . And the bispecific peptide was labeled with near-infrared (NIR) fluorochrome ICG-Der-02 for in vivo tumor EGFR and HER2 targeting. Afterwards, the EGFR and HER2 specificity of the fluorescent probe was tested in vitro for receptor binding assay and fluorescence microscopy and in vivo for subcutaneous MDA-MB-231 tumor targeting. The results indicated that the bispecific peptide had a high affinity to EGFR and HER2. Besides, in vitro and in vivo tumor targeting experiment indicated that the ICG-Der-02-( bispecific peptide) showed excellent tumor activity accumulation. Noninvasive NIR fluorescence imaging is able to detect tumor EGFR and HER2 expression based upon the highly potent bispecific peptide probe.
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Li Ding, Wei R. Chen, and Yueqing Gu "A bispecific peptide based near-infrared probe for in vivo tumor diagnosis", Proc. SPIE 8582, Biophotonics and Immune Responses VIII, 85820T (22 February 2013);

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