22 February 2013 Development of a QDots 800 based fluorescent solid phantom for validation of NIRF imaging platforms
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Abstract
Over the past decade, we developed near-infrared fluorescence (NIRF) devices for non-invasive lymphatic imaging using microdosages of ICG in humans and for detection of lymph node metastasis in animal models mimicking metastatic human prostate cancer. To validate imaging, a NIST traceable phantom is needed so that developed “first-inhumans” drugs may be used with different luorescent imaging platforms. In this work, we developed a QDots 800 based fluorescent solid phantom for installation and operational qualification of clinical and preclinical, NIRF imaging devices. Due to its optical clearance, polyurethane was chosen as the base material. Titanium dioxide was used as the scattering agent because of its miscibility in polyurethane. QDots 800 was chosen owing to its stability and NIR emission spectra. A first phantom was constructed for evaluation of the noise floor arising from excitation light leakage, a phenomenon that can be minimized during engineering and design of fluorescent imaging systems. A second set of phantoms were constructed to enable quantification of device sensitivity associated with our preclinical and clinical devices. The phantoms have been successfully applied for installation and operational qualification of our preclinical and clinical devices. Assessment of excitation light leakage provides a figure of merit for “noise floor” and imaging sensitivity can be used to benchmark devices for specific imaging agents.
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Banghe Zhu, Banghe Zhu, Eva M. Sevick-Muraca, Eva M. Sevick-Muraca, } "Development of a QDots 800 based fluorescent solid phantom for validation of NIRF imaging platforms", Proc. SPIE 8583, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue V, 858302 (22 February 2013); doi: 10.1117/12.2002098; https://doi.org/10.1117/12.2002098
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