Calibration of fluorescence reflectance reference phantoms

Jean-Pierre Bouchard; François Baribeau; Ozzy Mermut

doi:10.1117/12.2011346

28 February 2013 Calibration of fluorescence reflectance reference phantoms

Jean-Pierre Bouchard, François Baribeau, Ozzy Mermut

Proceedings Volume 8583, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue V; 85830K (2013) https://doi.org/10.1117/12.2011346
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Fluorescence intensity is often standardized by comparing the unknown sample signal to that of a reference solution with a known concentration of a reference fluorophore. To use this technique for in vivo fluorescence reflectance measurements standardization, a reference sample that also mimics the scattering and absorption properties of the tissue would need to be used. A simpler approach to fluorescence reflectance measurements standardization is to express the intensity of the measured fluorescence as a ratio of the excitation irradiance to the fluorescence radiance. This ratio of radiometric quantities can be measured by normalizing the measured fluorescence image to a reflectance image acquired with a reflection standard at the excitation wavelength (without the emission filter). Instruments could be calibrated to report their intensity results using this dimensionless ratio. The calibration could also be transferred from a reference calibrated instrument to other instruments through the use of fluorescence phantoms. Reporting fluorescence intensity measurements using this dimensionless ratio will ease instrument standardization and comparison of fluorescence reflectance results between instruments, vendors and applications.

Citation Download Citation

Jean-Pierre Bouchard, François Baribeau, and Ozzy Mermut "Calibration of fluorescence reflectance reference phantoms", Proc. SPIE 8583, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue V, 85830K (28 February 2013); https://doi.org/10.1117/12.2011346

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Reflectivity

Calibration

Optical filters

Cameras

Image filtering

Diffuse reflectance spectroscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years